Hosuk:LabNotes/2013-10-29

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Revision as of 21:51, 29 October 2013 by >Hosuki78
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Stage adapter detachment and reinstall test

  • Use 10/22 sample, which was used for the experiment yesterday (10/28)


Procedure

  • Image – 3 channel along Z stacking
  • Save location (Mark and find function)
  • Lower objective, detach stage adapter, move stage to left side far from original position
  • Reinstall adapter
  • Move stage back to saved position, and image


Result

  • 7 pixel, 281.25nm x 7 = 1.96875um off to upper direction.
  • There was no offset at other direction.
  • Composite image, Left:Before, Right:After (Blue : 1st rolony, Red : ACTB, Green : RAB7A)

File:DetachTest Before(left) After(Right) Red(AACTB) Green(RAB7A) Blue(1stRolony).png File:DetachTest Zoonin.png