Hosuk:LabNotes/2013-10-29
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Stage adapter detachment and reinstall test
- Use 10/22 sample, which was used for the experiment yesterday (10/28)
Procedure
- Image – 3 channel along Z stacking
- Save location (Mark and find function)
- Lower objective, detach stage adapter, move stage to left side far from original position
- Reinstall adapter
- Move stage back to saved position, and image
Result
- 7 pixel, 281.25nm x 7 = 1.96875um off to upper direction.
- There was no offset at other direction.
- Composite image, Left:Before, Right:After (Blue : 1st rolony, Red : ACTB, Green : RAB7A)
File:DetachTest Before(left) After(Right) Red(AACTB) Green(RAB7A) Blue(1stRolony).png File:DetachTest Zoonin.png