Hosuk:FISSEQ Primer information

From ZhangLabWiki
Revision as of 18:51, 12 November 2013 by >Hosuki78
Jump to navigation Jump to search

Primers

  • FISSEQ_RT : RT primer for 1st Rolony fabrication
    • /5Phos/TCTCGGGAACGCTGAAGANNNNNN


  • FISSEQ_RCA : RCA primer for 1st Rolony fabrication
    • TCTTCAGCGTTCCCGA*G*A


  • FISSEQ_ppRCA : RCA primer for 2nd Rolony fab. from padlock probes
    • GATATCGGGAAGCTGA*A*G


Padlock Probes

  • ppACTB
    • /5Phos/CTGTGCTCGCGGGGCGCTTCAGCTTCCCGATATCCGACGGACGTATCGGTAGTCGCAACGCAGGCAAAGGCGAGGCT


  • ppRAB7A
    • /5Phos/GAAGCGAGAAGGTCCAAGTTCTGCTTCAGCTTCCCGATATCCGACGGGTCTTGCGTGCGATACGGAGTAGAGGAGACTAAACGGAGGACA


  • ppMALAT1
    • /5Phos/TTTCTGCCTTTACTTATCAATTCCTTCAGCTTCCCGATATCCGACGGTCTACTTCGTCGCGTCAGACCAAATGGAGGTATGACATATAATCT


Detection Probes

  • FISSEQ_Adpt : Cy3 labeled probe for 1st Rolony detection
    • /5Cy3/TCTCGGGAACGCTGAAGA


  • FISSEQ_AdptCy5 : Cy5 labeled probe for 1st Rolony detection
    • /5Cy5/TCTCGGGAACGCTGAAGA


  • ACTB_1stRolonyFISH : ATTO488 labeled probe for ACTB on 1st Rolony
    • GGCAAAGGCGAGGCT/3ATTO488N/


  • RAB7A_1stRolonyFISH : ATTO550 labeled probe for RAB7A on 1st Rolony
    • GAGGAGACTAAACGGAGGACA/3ATTO550N/
  • Shifted FISSEQ_2ndRCAprimerDye : 2nd Rolony from all padlock probes
    • GGATATCGGGAAGCTGAA/3ATTO488N/


  • dcProbe0-Cy3 : for 2nd Rolony of ppACTB
    • Cy3-ACGTATCGGTAGTCGCAACGCA


  • dcProbe1-Cy3 : for 2nd Rolony of ppRAB7A
    • Cy3-GTCTTGCGTGCGATACGGAGTA


  • dcProbe2-Cy3 : for 2nd Rolony of ppMALAL1
    • Cy3-TCTACTTCGTCGCGTCAGACCA


  • RT-dT50 : Cy3 labeled probe for mRNA detection
    • /5Cy3/TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT


  • RT-Adapt : Cy5 labeled probe for cDNA after RT
    • /5Cy5/TCTTCAGCGTTCCCTAGA