Hosuk:LabNotes/2013-11-12

From ZhangLabWiki
Revision as of 00:25, 13 November 2013 by >Hosuki78 (Created page with "*LabNote ===Experimental Plan=== ====RT with 8 different RT probes==== *for RT from a specific location *Will be started 11/12 evening ~ finished at 11/...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search


Experimental Plan

RT with 8 different RT probes

  • for RT from a specific location
  • Will be started 11/12 evening ~ finished at 11/14 (Th)
  • Pick the best wells out of 32 wells


Padlock probe concentration

  • 2uM , 0.66uM was working
  • 1nM  12x10^10 numbers
  • MALAT1 was roughly 3700 numbers in one field of view (1mm^2)  ~ 654,900 in one MatTek dishes
  • --> if there are 100 targets in Rolony  6.5 x 10^6 targets
  • 0.1nM, 1nM, 10nM, 100nM, 1uM , 5uM for 1 padlock probes  MALAT1 pp
  • 1 MatTek Dish


Ampligase test

  • Just Do 2nd RCA  2 MatTek Dishes
    • Try 2nd RCA, and anneal 2nd RCA detection probes
    • Use 3 padlock probes
    • Parameters
    • Temperature : 55C
    • Time : 4hr
    • Enzyme concentration : 5U (1uL of Ampligase)


  • Optimize --> later