Daniel:Notebook/HiResChrPaint/2013-11-15

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Revision as of 02:14, 15 November 2013 by >Djacobse (Created page with "=Positive Control (Started 11/14/2013)= Back to Calendar ==Lambda Exonuclease Digestion== 1....")
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Positive Control (Started 11/14/2013)

Back to Calendar

Lambda Exonuclease Digestion

1. Reaction table
Sample V6S1AB V6S1CD V6S2AB V6S2CD
ng/uL 128.6 125.8 147.2 150
ug in 25 uL 3.2 3.1 3.7 3.8
uL added 25 25 25 25
uL 10x buffer 5 5 5 5
uL Lambda Exonuclease (5U/uL) 3 3 3 3
uL nfH2O 17 17 17 17
total 50 50 50 50
2. Incubate for 1 hour at 37C followed by 10 minute heat inactivation at 75C
3. Pool samples
4. ssDNA column purification, elute with 20 uL nfH2O
5. Measure Concentration in Nanodrop