Daniel:Notebook/HiResChrPaint/2013-11-16

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Revision as of 01:38, 16 November 2013 by >Djacobse (→‎Lambda Exonuclease Digestion)
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Positive Control 2 (V6S3 & V6S4, Started 11/15/2013)

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Lambda Exonuclease Digestion

1. Reaction table
Sample V6S3AB V6S3CD V6S4AB V6S4CD
ng/uL 169.9 180 169.4 180.1
ug in 25 uL 4.2 4.5 4.2 4.5
uL added 25 25 25 25
uL 10x buffer 5 5 5 5
uL Lambda Exonuclease (5U/uL) 3 3 3 3
uL nfH2O 17 17 17 17
total 50 50 50 50
2. Incubate for 1 hour at 37C followed by 10 minute heat inactivation at 75C
3. Pool samples
4. ssDNA column purification, elute with 20 uL nfH2O
5. Measure Concentration in Nanodrop