Hosuk:LabNotes/2013-11-19
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Dye labeled Probe Annealing Test – with Cy3-1st Rolony
- Figure out upper limit by Formamide concentration
Procedure
- 2048 x 2-48, 600Hz Image quality is not different from 400Hz.
- Sample : MatTek Dish, made at 07/09
- Each stripping, and annealing : 15min incubation @ RT
- Process Steps
- Step 0 : Strip
- Step 1 : Cy5 in 2x SSC
- Step 2 : Strip
- Step 3 : Cy5 in 70% Formamide + 2x SSC
- Step 4 : Strip
- Step 5 : Cy5 in 10% Formamide + 2x SSC
- Step 6 : Strip
- Step 7 : Cy5 in 30 % Formamide + 2x SSC
- Step 8 : Strip
- Step 9 : Cy5 in 50% Formamide + 2x SSC
- Step 10 : Strip
- Step 11 : Cy5 in 70% Formamide + 2x SSC