Arichard:Notebook/2013/December

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Revision as of 21:22, 4 December 2013 by >Andrew (→‎12/03/2013)
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December, 2012

12/02/2013

Meeting with Ben Siddoway and Gwen Kaeser.

Discussed possible quality control measures.

  • Neuronal nuclei come from three brain banks:
    • UC Irvine
    • Maryland
    • Wash U

12/03/2013

Shadowed Gwen for neuronal nuclei isolation

[Protocol used]

We decided to use the same samples, but take samples along the way for bulk prep (Nextera). Female preferred so that presence of Y chromosome can be used as a marker for contamination by myself.

Bulk sorted 3 types, all NeuN+

  • 1864+, female Down's from ???.
  • 1568+, female AD from Maryland.
  • 61788+, female AD from Wash U. (New patient)

Notes

  • Started with 0.095 g 1568, 0.14 g 61788, and 0.3 g 1864.
  • Filtered with 40 um BD Falcon filter.
  • Fixed 25 min, not 30.

Time point samples (3) for bulk prep on each sample (3) (9 total):

  • 1 = After filter
  • 2 = Before FACS
  • 3 = After FACS