AlanFung:LabNotes/Capturing/2014-1-2
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Helping Dheeraj to test Klentaq capture reactions
- Dheeraj reached out to me saying he wants to start a qPCR reaction on the Klentaq capture reaction, he was working with Noi to test the protocol, but Noi is sick and I will be helping to perform the qPCR
- They have 12 samples and we will be amplifying the captured samples with qPCR using the same protocol as it in Stoffol capture
Protocol
PCR amplification to check if capture works
Components | 1x rxn (ul) | 12x rxn (ul) | Final Conc. |
Circularized DNA template | 2.5 | 0 | |
KAPA SSYBR FAST MM 2X | 12.5 | 150 | 1X |
10uM AmpF6.4Sol | 0.5 | 6 | 200nM |
10uM AmpR6.3IndX | 0.5 | 6 | 200nM |
H2O | 10 | 120 | |
Total | 25 |