Daniel:Notebook/HiResChrPaint/2014-1-10
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Alu Control (Started 1/08/2014)
Production qPCR
Since I didn't make enough the first time, I'm going to do another production PCR to make more Alu probe.
1. Reaction Table
gDNA | 2X Kapa SYBR qPCR MM | 100 uM AP1V6-Am | 100 uM AP2V6 | H20 | Total Volume | |
per rxn | 1 | 50 | 0.5 | 0.5 | 48 | 100 |
PGP1F (12.1X) | 12.1 | 605 | 6.05 | 6.05 | 580.8 | 1210 |
2. Aliquot 100 uL into each of 3 qPCR tubes 3. PCR protocol: i. PCR 3 min 95C ii. 95C 10 sec iii. 55C 30 sec iv. 72C 20 sec v. plate read vi. goto ii x30 vii. 72C 2 min viii. 16C hold 4. Pool 3 samples together each 5. Column Purification, elute with 40 uL EB 6. Measure concentrations in nanodrop