Daniel:Notebook/HiResChrPaint/2014-1-14

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Revision as of 17:48, 14 January 2014 by >Djacobse (Created page with "=Alu Control (Started 1/08/2014)= Back to Calendar ==Dye Coupling Test (with Positive Control)==...")
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Alu Control (Started 1/08/2014)

Back to Calendar

Dye Coupling Test (with Positive Control)

Repeating the dye coupling test one last time using a positive control as well.

Samples

  • V6S3-A; 13.5 uL, total 1.0 ug
  • Alu2-D; total 1.0 ug
  • Human Cot-1 DNA, 1 ug
0. Vaccuum centrifuge DNA; resuspend in 2.5 uL nfH2O
1. Warm DMSO and bicarbonate labeling buffer and nuclease free water to room temperature
2. Add 1.5 uL of sodium bicarbonate buffer
3. Denature samples for 5 minutes at 95C, then snap cool using ice box
4. Dissolve 1 vial of reactive dye in 2 uL solvent; vortex ≥10 seconds
   *dye cannot be saved for later use.  Use immediately!
5. Add 1 uL dye to sample
6. Incubate in the dark for 1 hour
7. Add 20 uL nfH20 to sample; Measure concentration in Nanodrop
8. Centri-Sep column purification
9. Measure concentration in Nanodrop