Matt:LabNotes/2014-1-17

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Revision as of 03:34, 28 January 2014 by >Mzcai (→‎Testing non-specific binding of Shifted FISSEQ)
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Testing non-specific binding of Shifted FISSEQ[edit]

Procedure[edit]

Using negative controls (E7 & F7) from Matt:LabNotes/2014-1-15

  1. Strip with 80% formamide for 10min @50C
    1. Rinse twice with H2O
  2. Image 488 channel
  3. Add 50ul 2uM Shifted FISSEQ preheated to 60C (1ul 200uM Dye + 30ul 100% formamide + 10ul 20X SSC + 59ul H2O)
    1. Incubate RT for 15min
    2. Wash 2 times with 2X SSC
  4. Image 488 channel

Results[edit]