Matt:LabNotes/2014-1-31
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Shifted FISSEQ vs FISSEQ_Adpt Signal Count in Same Wells
- Previously compared the two dyes in adjacent wells Matt:LabNotes/2014-1-28
- Here I strip FISSEQ_Adpt from before and add Shifted FISSEQ to column 9
- Put Shifted FISSEQ_2ndRCAprimerDye in columns 4 and 9
- Put RAB7A_1stRolonyFISH in columns 3 and 10
C9_488: 129
D9_488: 203
E9_488: 842
F9_488: 14,654 (2nd Try)
RT_RAB7A Primers and the Number of RAB7A Primary Rolonies
- Using RAB7A_1stRolonyFISH (ATTO550)
C10_550: 24 D10_550: 24 E10_550: 13 F10_550: 35