Daniel:Notebook/HiResChrPaint/2014-2-13
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Multi-Color FISH (Started 02/08/2014)
Cot1 Labeling (Started 02/12/2014)
- Remove samples from -80C and centrifuge for 20 minutes at 12000 RPM at 4 C
- Remove supernatant and replace with 750uL 75% EtOH
- Centrifuge for 10 minutes at 12000 RPM at 4 C
- Remove supernatant and leave in hood to dry
- Resuspend each sample in 20 uL nfH2O
- Measure concentration in nanodrop
- Run on TBE gel to ensure correct size distribution
- Pool all high MW samples and all low MW samples
Results
Nanodrop Results
High MW | A | B | C | D | Low MW | A | B | C | D |
ng/uL | 10.3 | 44.8 | 13.6 | 10.1 | 17.7 | 20.4 | 22.5 | 70.9 | |
ug in 20 uL | 0.2 | 0.9 | 0.3 | 0.2 | 0.4 | 0.4 | 0.5 | 1.4 | |
Total | 1.6 | 2.6 |
Gel Image