Daniel:Notebook/HiResChrPaint/2014-2-9
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Multi-Color FISH (Started 02/08/2014)[edit]
Cot1 Labeling (Started 02/08/2014)[edit]
- Remove samples from -80C and centrifuge for 20 minutes at 12000 RPM at 4 C
- Remove supernatant and replace with 750uL 75% EtOH
- Centrifuge for 10 minutes at 12000 RPM at 4 C
- Remove supernatant and leave in hood to dry
- Resuspend each sample in 20 uL nfH2O
- Measure concentration in nanodrop
- Run on TBE gel to ensure correct size distribution
- Pool all high MW samples and all low MW samples
Results[edit]
Nanodrop Results
High MW | Low MW | |
ng/uL | 7.3 | 8 |
ug in 50 uL | 0.4 | 0.4 |
Gel Image