Kun:LabNotes/ASE/2007-11-9
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Exp.1 eSNPs capture (cont.)
Perform PCR on 5ul capturing reactions.
x 1 x 12 template: 5ul each ABI 10x buffer: 4.5ul 54ul 25mM MgCl2: 3ul 36ul 10mM dNTP: 1ul 12ul 100uM AmpF2Sol: 0.15ul 1.8ul 100uM AmpRSol: 0.15ul 1.8ul 50X SYBG I: 0.4ul 4.8ul iTaq: 0.5ul 6ul H2O: 35.3ul 430ul
94C 3min -> 10 cycles of (94C 45sec -> 56C 2min -> 72C 1min) -> 20 cycles of (94C 45 sec -> 72C 1min) -> 72C 3min -> 15C hold.