Hosuk:LabNotes/2014-2-18
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NCIH1975 : Mutation Detection : Data Analysis
Procedure
- Make one image from all 8 images by Maximum intensity projection , and Run PISA
- Extract location information from the BW image(--> mask image)
- Look all 8 images based on the location info from mask image, Decode Rolony signals
- Set threshold (9000 or 10000), and search range : 9 pixel or 11 pixel (--> 4, or 5 pixel from the center position)
- Most of rolony signals have above 20000 intensity and backgound have below 7000~8000
Result
Compare prev. method and current method
- Different counts and positions for each target
File:Compare Method1-0208 Method2-0217.png
Target counts at different parameters
- Different Threshold and search range values generated different counts as well as different positions...
- Which one would be correct?...
File:AnalysisParams Threshold SearchRange 4Tests Counts.png File:AnalysisParams Threshold SearchRange 4Tests CompositeImage Pos2.png