Daniel:Notebook/HiResChrPaint/2014-2-16

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Revision as of 18:22, 2 July 2014 by >Djacobse
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Multi-Color FISH (Started 02/08/2014)[edit]

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Cot1 Labeling (Started 2/14/2014)[edit]

  1. Remove samples from -80C and centrifuge for 20 minutes at 12000 RPM at 4 C
  2. Remove supernatant and replace with 750uL 75% EtOH
  3. Centrifuge for 10 minutes at 12000 RPM at 4 C
  4. Remove supernatant and leave in hood to dry
  5. Resuspend each sample in 20 uL nfH2O
    1. Leave overnight, continued tomorrow