Matt:LabNotes/2014-2-21

From ZhangLabWiki
Revision as of 03:37, 22 February 2014 by >Mzcai (Created page with "==Screening 32 wells for Ampligase Positive Control Test== *Using 32 wells of 96-well plate Matt:LabNotes/2014-1-24 *Used microscope to check cell density and morphology...")
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Screening 32 wells for Ampligase Positive Control Test

  • Used microscope to check cell density and morphology in each well to pick the best wells for this experiment
    • Color coded each well based on quality of cells
    • Designated 2 or 4 wells for each sample group
      • All wells will have 0.5 U Ampligase/ul except for 2 Fixed Template wells

File:32wellSamples.JPG

Checking MALAT1 Targets in 1000nt RT_RAB7A

  • In wells: F9 and F10

Checking for non-specific binding of dcProbe2RevComp-Cy3

  • In wells: F7 and F8

Checking MALAT1, RAB7A, and ACTB Targets in RandomHex Primary Rolonies

  • In wells: E6 and F6