Matt:LabNotes/2014-2-21
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Screening 32 wells for Ampligase Positive Control Test
- Using 32 wells of 96-well plate Matt:LabNotes/2014-1-24
- Used microscope to check cell density and morphology in each well to pick the best wells for this experiment
- Color coded each well based on quality of cells
- Designated 2 or 4 wells for each sample group
- All wells will have 0.5 U Ampligase/ul except for 2 Fixed Template wells
Checking MALAT1 Targets in 1000nt RT_RAB7A
- In wells: F9 and F10
Checking for non-specific binding of dcProbe2RevComp-Cy3
- In wells: F7 and F8
Checking MALAT1, RAB7A, and ACTB Targets in RandomHex Primary Rolonies
- In wells: E6 and F6