Daniel:Notebook/HiResChrPaint/2014-2-24

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Revision as of 18:27, 2 July 2014 by >Djacobse
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Multi-Color FISH (Started 02/08/2014)[edit]

Back to Calendar

Hybridization (Started 02/23/2014)[edit]

Day 2

  1. The next day, remove coverslip carefully and wash in pre-warmed 2X SSCT at 60C for 15 minutes
  2. Incubate in 2X SSCT at room temperature for 10 minutes
  3. Incubate in 0.2X SSC at room temperature for 10 minutes
  4. Dry with kimwipes; do not let the surface containing the cells touch the kimwipes
  5. Add 15 uL DRAQ5 (diluted 1:1000) to center of dish
  6. Cover with 22x22 #1.5 coverslip; seal with nail polish
    1. Allow at least 30 minutes for nail polish to dry
    2. Slides are quite stable for several months at 4C
  7. Waited 2 hours before going to microscope room

Microscopy[edit]

Imaged samples B,C, and D, but didn't have time for sample A (V6S3-546 only). See tomorrow for continued microscopy results.