Song Chen:Daily/NOTES/2014-2-22
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Cell density and DE differentiation
Setting: Expose 1209 ms; Gain 5; Sharp 20; CERC epi-fluorescent microscope.
1x seeding density | 3x seeding density | 10x seeding density |
File:022214 H9DE 1x SOX17.jpg | File:022214 H9DE 3x SOX17.jpg | File:022214 H9DE 10x SOX17.jpg |
File:022214 H9DE 1x DAPI.jpg | File:022214 H9DE 3x DAPI.jpg | File:022214 H9DE 10x DAPI.jpg |
DE differentiation condition: D1 ActA, D2-4 ActA+0.2%FBS 1x seeding density: D6-D7 hES passage at 1:12-1:15 ratio High seeding density results in high efficient DE differentiation