Song Chen:Daily/NOTES/2014-2-22

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Revision as of 02:37, 25 February 2014 by >Chensong
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Cell density and DE differentiation

Setting: Expose 1000 ms; PFBH 406, 10x obj, SOX17/DAPI

1x seeding density 3x seeding density 10x seeding density
File:022214 H9DE 1x SOX17.png File:022214 H9DE 3x SOX17.png File:022214 H9DE 10x SOX17.png
File:022214 H9DE 1x DAPI.png File:022214 H9DE 3x DAPI.png File:022214 H9DE 10x DAPI.png

DE differentiation condition: D1 ActA100, D2-4 ActA100+0.2%FBS

1x seeding density: D6-D7 hES passage at 1:12-1:15 ratio

High seeding density results in high efficient DE differentiation


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