Song Chen:Daily/NOTES/2014-2-21

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Hepatic commitment from high-density DE differentiation

Setting: Expose 1000 ms, PFBH 406, 10x obj, AFP/HNF4A; AFP/HNF6; AFP/PROX1;

AFP/HNF4A AFP/HNF6 AFP/PROX1
File:022114 H9DE AFP.png File:022114 H9DE AFP.2.png File:022114 H9DE AFP.3.png
File:022114 H9DE HNF4A.png File:022114 H9DE HNF6.png File:022114 H9DE PROX1.png

DE differentiation condition: D1 ActA100, D2-4 ActA100+0.2%FBS

High seeding density: D6-D7 hES passage at 1:1.2-1:1.5 ratio

Successful hepatic differentiation of high-density DE differentiated cells


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