Revision as of 23:07, 5 March 2014 by >Alan6017518
Secondary Rolony Generation for Padlock Probe Concentration Test
Samples
- 96 well plate containing NCIH-1975 cells fixed by Hosuk
- Location and save location of 12 position in 96 well plate for the experiment
- High Concentration(100pM)-
Anneal Padlock Probes for 24 hours
- 1X Ampligase Buffer
- Various padlock probe concentration
- 0.5 U/ul Ampligase
- Incubate @ 45C for 24 hours
- Wrapped in parafilm and put in ziploc with wet towel
Group 1 100pM ppMALAT1
Group 1
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Group 2
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Group 3
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Image
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Well
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Mark & Find
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Image
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Well
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Mark & Find
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Image
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Well
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Mark & Find
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A
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C3
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1
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E
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F8
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15
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I
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C6
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9
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B
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D3
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2
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F
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F5
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14
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J
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C7
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10
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C
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F3
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13
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G
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F6
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7
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K
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E7
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11
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D
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C5
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4
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H
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E6
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8
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L
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F7
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12
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1
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2
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3
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4
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5
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6
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7
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8
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9
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10
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11
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12
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A
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B
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C
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C3
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C5
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C6
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C7
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D
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D3
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E
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E6
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E7
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F
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F3
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F5
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F6
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F7
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F8
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G
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H
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File:Ppconcentration NCHI1975 Group1.jpg
Group 2 10pM ppMALAT1
Group 2
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Image
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Well
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Mark & Find
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E
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F8
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15
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F
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F5
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14
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G
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F6
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7
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H
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E6
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8
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File:Ppconcentration NCHI1975 Group2.jpg
Group 3 0.1pM ppMALAT1
Group 3
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Image
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Well
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Mark & Find
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I
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C6
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9
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J
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C7
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10
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K
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E7
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11
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L
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F7
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12
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File:Ppconcentration NCHI1975 Group3.jpg