Daniel:Notebook/HiResChrPaint/2014-3-5

From ZhangLabWiki
Revision as of 23:53, 5 March 2014 by >Djacobse (→‎qPCR Results)
Jump to navigation Jump to search

Overload FISH

Back to Calendar

From the results last week, I believe that the probe sets may need an increased signal in order to be visualized. In that spirit, I'm going to amplify more probe and use a lot of it on one experiment.

qPCR

  10 nM OligoPool 2X Kapa SYBR qPCR MM 100 uM AP1V61U 100 uM AP2V6 2 mM aa-dUTP H20 Total Volume
per rxn 0.4 50 0.4 0.4 2.5 46.3 100
V6S2 (6.1x) 2.44 305 2.44 2.44 15.25 282.43 610
V6S3 (6.1x) 2.44 305 2.44 2.44 15.25 282.43 610

qPCR Results

File:2014-03-05-V6S2V6S3.png

Nanodrop Results