Daniel:Notebook/HiResChrPaint/2014-3-5
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Overload FISH
From the results last week, I believe that the probe sets may need an increased signal in order to be visualized. In that spirit, I'm going to amplify more probe and use a lot of it on one experiment.
qPCR
10 nM OligoPool | 2X Kapa SYBR qPCR MM | 100 uM AP1V61U | 100 uM AP2V6 | 2 mM aa-dUTP | H20 | Total Volume | |
per rxn | 0.4 | 50 | 0.4 | 0.4 | 2.5 | 46.3 | 100 |
V6S2 (6.1x) | 2.44 | 305 | 2.44 | 2.44 | 15.25 | 282.43 | 610 |
V6S3 (6.1x) | 2.44 | 305 | 2.44 | 2.44 | 15.25 | 282.43 | 610 |