Daniel:Notebook/HiResChrPaint/2014-3-5

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Revision as of 01:53, 6 March 2014 by >Djacobse (→‎Nanodrop Results)
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Overload FISH

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From the results last week, I believe that the probe sets may need an increased signal in order to be visualized. In that spirit, I'm going to amplify more probe and use a lot of it on one experiment.

qPCR

  10 nM OligoPool 2X Kapa SYBR qPCR MM 100 uM AP1V61U 100 uM AP2V6 2 mM aa-dUTP H20 Total Volume
per rxn 0.4 50 0.4 0.4 2.5 46.3 100
V6S2 (6.1x) 2.44 305 2.44 2.44 15.25 282.43 610
V6S3 (6.1x) 2.44 305 2.44 2.44 15.25 282.43 610

qPCR Results

File:2014-03-05-V6S2V6S3.png

Nanodrop Results

Sample V6S2A V6S2B V6S3A V6S3B
ng/uL DNA 78.9 77.5 85.2 86.2
ug in 40 uL 3.2 3.1 3.4 3.4
ug total   6.3   6.9