Kun:LabNotes/CpgSeq/2007-11-9

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Revision as of 23:31, 9 November 2007 by KunZhang (talk | contribs) (New page: ===Exp. 1 Perform PCR on the bisulfite converted DNAs (positive control)=== *Template: Jurkat DNA, DNA RL, concentration unknown. *Primer: hd48MIP-1, hd48MIP-2, hd48MIP-5. ...)
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Exp. 1 Perform PCR on the bisulfite converted DNAs (positive control)

  • Template: Jurkat DNA, DNA RL, concentration unknown.
  • Primer: hd48MIP-1, hd48MIP-2, hd48MIP-5.
                            x1         x6
        template:           1ul       each
        10x ABI buffer:   2.5ul       15ul
        25mM MgCl2:       1.5ul        9ul
        10mM dNTP:        0.5ul        3ul
        10uM Primer mix:  0.5ul      1ulx3 
        50X SYBG I:       0.2ul      1.2ul
        iTaq:             0.2ul      1.2ul
        H2O:             18.6ul      114ul

94C 3min -> 40 cycles of (94C 30sec -> 57C 30min -> 72C 1min) -> 72C 3min -> 15C hold.