Kun:LabNotes/CpgSeq/2007-11-9
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Exp. 1 Perform PCR on the bisulfite converted DNAs (positive control)
- Template: Jurkat DNA, DNA RL, concentration unknown.
- Primer: hd48MIP-1, hd48MIP-2, hd48MIP-5.
x1 x6 template: 1ul each 10x ABI buffer: 2.5ul 15ul 25mM MgCl2: 1.5ul 9ul 10mM dNTP: 0.5ul 3ul 10uM Primer mix: 0.5ul 1ulx3 50X SYBG I: 0.2ul 1.2ul iTaq: 0.2ul 1.2ul H2O: 18.6ul 114ul
94C 3min -> 40 cycles of (94C 30sec -> 57C 30min -> 72C 1min) -> 72C 3min -> 15C hold.