Song Chen:Daily/NOTES/2014-3-6
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hES change medium:
- PGP iPS Brainbow knockin #1, #2-4 and #2-6 p2, 2 well*6 well plate in hES/NHSM, respectively;
- H9 p62 2*6 cm+24 well*48-well plate in NHSM
- H9 Bb6 2 well*6 well plate
- PGPs iPS LCB+CRE p62 1*6 cm in hES medium
send H9 in NHSM:
- 1*T25 flask to UCLA via Fedex(800555872882)
DE differentition(H9 p62, for methylation analysis-Dinh):
- 3 well*6-well plate, hES at d2, ActA100 d4
check default GFP expression in knockin iPS lines:
Setting: Expose 1209 ms, Cerc, 20x obj,GFP/Brightfield, KI(knockin)
Knockin iPS cell lines don't express default GFP in every cells as expected. #2-4 has no detectable GFP expression, while #1 and #2-6 have a few GFP positive cells. And when compared with brainbow random integrated cell line #6, GFP is expressed at a relative low level, which probably due to the nature of single copy insertion of knockin. The possibility of fluctuation in brainbow expression between knockin cells or contamination with non-transgenic iPS cells await further verification.