Daniel:Notebook/HiResChrPaint/2014-3-13
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Overload FISH (Started 3/5/2014)
Report
Sample Matrix
Sample | Sample A | uL sample A (pmol) | Sample B | uL sample B (pmol) | Sample B | uL sample B (pmol) |
V6S3 +V6S2 (A) | V6S3 | 40 (48) | V6S2 | 20 (98) | Unlabeled Cot1 | 2 (2 ug) |
V6S3+Cot1 (B) | V6S3 | 40 (48) | Cot1 | 20 (10) | ||
BSPP Negative Control (D) | BSPP1B | 5 (10) | Unlabeled Cot1 | 2 (2 ug) |
Sample C (BSPP1)
This sample is just BSPP1 labeled with A-488. As we expect, there is not a lot of nuclear signal, and it is in no particular patterning.
File:20140310 BSPP1 ManyNucleii1.jpg|File:20140310 BSPP1 ManyNucleii2.jpg| | |
Nucleii from BSPP1 sample, 63x objective, 1x zoom. Draq5 in blue, Alexa 488 in green |