Daniel:Notebook/HiResChrPaint/2014-3-13

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Revision as of 21:29, 13 March 2014 by >Djacobse (Created page with "=Overload FISH (Started 3/5/2014)= Back to Calendar ==Report== '''Sample Matrix''' {| class="wi...")
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Overload FISH (Started 3/5/2014)

Back to Calendar

Report

Sample Matrix

Sample Sample A uL sample A (pmol) Sample B uL sample B (pmol) Sample B uL sample B (pmol)
V6S3 +V6S2 (A) V6S3 40 (48) V6S2 20 (98) Unlabeled Cot1 2 (2 ug)
V6S3+Cot1 (B) V6S3 40 (48) Cot1 20 (10)    
BSPP Negative Control (D) BSPP1B 5 (10) Unlabeled Cot1 2 (2 ug)    


Sample C (BSPP1)

This sample is just BSPP1 labeled with A-488. As we expect, there is not a lot of nuclear signal, and it is in no particular patterning.

File:20140310 BSPP1 ManyNucleii1.jpg|File:20140310 BSPP1 ManyNucleii2.jpg|
Nucleii from BSPP1 sample, 63x objective, 1x zoom. Draq5 in blue, Alexa 488 in green