Dinh/Dinh 2014/NOTES/2014-3-11

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Revision as of 16:44, 19 March 2014 by >Dinh
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HOTSPOTS 460K Capture

  • Capture calculations
Size of Probes 90000 units
gDNA 150 ng
Human (MW) 1.82E+012 g/moles
1 Probe (MW) 31360.1 g/moles
Template 8.23E-011 nmoles
Probes(100:1) 8.23E-009 nmoles
Amount Probes needed 23.23 ng
Size of Probes 6553 units
gDNA 150 ng
Human (MW) 1.82E+012 g/moles
1 Probe (MW) 31360.1 g/moles
Template 8.23E-011 nmoles
Probes(100:1) 8.23E-009 nmoles
Amount Probes needed 1.69 ng
 Human MW = (3E109 bp)*(607.4 gmoles-1/bp)+157.9gmoles-1