Kun:LabNotes/ASE/2007-11-11
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Exp.1 eSNPs capture (cont.)
Perform PCR on 5ul the capturing reaction on Hues6 gDNA, compare iTaq with JumpStart Taq.
x 1 x 2 template: 5ul 10ul ABI 10x buffer: 4.5ul 9ul 25mM MgCl2: 3ul 6ul 10mM dNTP: 1ul 2ul 100uM AmpF2Sol: 0.15ul 0.3ul 100uM AmpRSol: 0.15ul 0.3ul 50X SYBG I: 0.4ul 0.8ul iTaq: 1ul 2x1ul H2O: 35.3ul 70.6ul
94C 3min -> 10 cycles of (94C 45sec -> 56C 2min -> 72C 1min) -> 10 cycles of (94C 45 sec -> 72C 1min) -> 72C 3min -> 15C hold.