Dinh/Dinh 2014/NOTES/2014-4-8
Jump to navigation
Jump to search
HOTSPOTS 460K sequencing library
- Indx 28, Indx 29 gel cut:
File:HOTSPOTS Indx28,Indx29 sizeselect.jpg
- Added three libraries with Index 33,35,37 from full HOTSPOTS 460K capture of GM12878, cut from top band.
- Pool sequencing library together, concentration is ~2.64 ng/ul
- Since Indx28 is only 1/5 of the total library (band at 450 bp), and 29,33,35,37 are at 510 bp, the average size is:
450*0.20 + 510*0.80 = 498 bp
- TBE Gel:
File:HOTSPOTS seqLib Apr9.png