Hosuk:LabNotes/2014-4-10
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Cancer Cell : Mutation detection test - 2nd Try
- NCIH-1975 in 24 well plate
- Fix : 04/08
- Finish all process 04/10
- Well 1, 2 : EGFR, BRAF RT Primers
- Well 3, 4 : EGFR, BRAF RT Primers + BRAF Stop primer 200bp
- Well 5, 6 : EGFR, BRAF RT Primers + BRAF Stop primer 200bp
- Well 7, 8, 9, 10 : random hexamer
Result for 1st Rolonies
- Well 1 ~ Well 6 have similar number of rolonies,
- which the numbers are much less than Well 7, 8, and 9.
- (Well 10 has few, it’s because of mistake at RNaseH step)