Hosuk:LabNotes/2014-4-10

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Revision as of 20:56, 10 April 2014 by >Hosuki78 (Created page with "*LabNote ===Cancer Cell : Mutation detection test - 2nd Try=== *NCIH-1975 in 24 well plate *Fix : 04/08 *Finish all process 04/10 *Well 1, 2 : EGFR, BR...")
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Cancer Cell : Mutation detection test - 2nd Try

  • NCIH-1975 in 24 well plate
  • Fix : 04/08
  • Finish all process 04/10


  • Well 1, 2 : EGFR, BRAF RT Primers
  • Well 3, 4 : EGFR, BRAF RT Primers + BRAF Stop primer 200bp
  • Well 5, 6 : EGFR, BRAF RT Primers + BRAF Stop primer 200bp
  • Well 7, 8, 9, 10 : random hexamer


File:24Well 2014-04-10.png


Result for 1st Rolonies

  • Well 1 ~ Well 6 have similar number of rolonies,
  • which the numbers are much less than Well 7, 8, and 9.
    • (Well 10 has few, it’s because of mistake at RNaseH step)