Noi/NOTES/2014-4-14
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gDNA extraction of cell cultures (Song' samples)
- Sample info.
- - Three vials for day 0 ESCs (ESCd0.1, ESCd0.2,ESCd0.3)
- - Three vials for definitive endoderm cells (DEd3.1,DEd3.2,DEd3.3)
- - Two vials for naive cells. (naiveESC.1, naiveESC.2)
- - Extract gDNA using DNeasy Blood & Tissue Kit (QIAGEN) with RNaseA treatment and followed the instruction of the kit
- - Elute DNA with 200ul AE buffer (single elution)
- - Measure concentration with Nanodrop (ND) and Qubit dsDNA HS assay
Qubit dsDNA HS assay and Nanodrop (ND) quantification
Sample | Conc. in the Qubit | Unit | uL used | Dilution | Sample conc. (ng/ul) | Yields in 200ul (ug) | conc. By ND (ng/ul) | 260/280 | 260/230 |
ESCd0.1 | 201 | ng/mL | 1 | 200 | 40.30 | 8.06 | 53.15 | 1.92 | 1.88 |
ESCd0.2 | 280 | ng/mL | 1 | 200 | 56.00 | 11.20 | 76.53 | 1.88 | 1.81 |
ESCd0.3 | 430 | ng/mL | 1 | 200 | 86.00 | 17.20 | 107.13 | 1.91 | 1.94 |
DEd3.1 | 170 | ng/mL | 1 | 200 | 34.00 | 6.80 | 44.91 | 1.91 | 2.07 |
DEd3.2 | 188 | ng/mL | 1 | 200 | 37.70 | 7.54 | 47.82 | 1.91 | 2.02 |
DEd3.3 | 129 | ng/mL | 1 | 200 | 25.70 | 5.14 | 34.79 | 1.84 | 1.85 |
naiveESC.1 | 132 | ng/mL | 1 | 200 | 26.30 | 5.26 | 39.75 | 1.86 | 1.55 |
naiveESC.2 | 150 | ng/mL | 1 | 200 | 30.00 | 6.00 | 44.82 | 1.86 | 1.52 |
- - For accuracy, the concentration will be based on Qubit measurement