Matt:LabNotes/2014-5-16

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Revision as of 01:41, 17 May 2014 by >Mzcai (Created page with "==Detecting ppMALAT1 Hybridization== *Use sample from Matt:LabNotes/2014-5-7 **A3, A4, & A5: Hybridize 10nM ppMALAT1 & 1uM dcProbe2-RevComp in one step **D3 & D4: Hybridi...")
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Detecting ppMALAT1 Hybridization

  • Use sample from Matt:LabNotes/2014-5-7
    • A3, A4, & A5: Hybridize 10nM ppMALAT1 & 1uM dcProbe2-RevComp in one step
    • D3 & D4: Hybridize 10nM ppMALAT1 for 24 hrs, 1uM dcProbe2-RevComp after
    • D5: Negative Ctrl (1uM dcProbe2-RevComp)

Procedure

  • Add 100ul of ppMALAT1 + dcProbe2-RevComp @45C for 24hrs (6:30p- )

A3-A5

Component Volume
10X Ampligase Buffer 30
2uM ppMALAT1 1.5
100uM dcProbe2-RevComp 3
H2O 265.5
Total 300

D3&D4

Component Volume
10X Ampligase Buffer 20
2uM ppMALAT1 1
H2O 179
Total 200

D5

Component Volume
10X Ampligase Buffer 10
100uM dcProbe2RevComp 1
H2O 89
Total 100