Matt:LabNotes/2014-5-16
Jump to navigation
Jump to search
Detecting ppMALAT1 Hybridization
- Use sample from Matt:LabNotes/2014-5-7
- A3, A4, & A5: Hybridize 10nM ppMALAT1 & 1uM dcProbe2-RevComp in one step
- D3 & D4: Hybridize 10nM ppMALAT1 for 24 hrs, 1uM dcProbe2-RevComp after
- D5: Negative Ctrl (1uM dcProbe2-RevComp)
Procedure
- Add 100ul of ppMALAT1 + dcProbe2-RevComp @45C for 24hrs (6:30p- )
A3-A5
Component | Volume |
10X Ampligase Buffer | 30 |
2uM ppMALAT1 | 1.5 |
100uM dcProbe2-RevComp | 3 |
H2O | 265.5 |
Total | 300 |
D3&D4
Component | Volume |
10X Ampligase Buffer | 20 |
2uM ppMALAT1 | 1 |
H2O | 179 |
Total | 200 |
D5
Component | Volume |
10X Ampligase Buffer | 10 |
100uM dcProbe2RevComp | 1 |
H2O | 89 |
Total | 100 |