Daniel:Notebook/HiResChrPaint/2014-6-16

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Revision as of 22:57, 16 June 2014 by >Djacobse (→‎Probe Mining)
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Probe Design

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Using the Oligopaints scripts by Beliveau, my goal is to design a positive/negative control for the FISH probes. The control is to focus on a region of the X chromosome, yet to be determined. Using male cells (such as PGP1), exactly one region should be painted per cell. This serves as a potential positive and negative control.

Probe Mining

Scripts:


Setting up the scripts for probe mining. I'm doing this locally on my machine for now, following the instructions in the two pdfs above.

Setting up a BLAST Database

Required for running the Oligopaints scripts.