Matt:LabNotes/2014-6-19

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Check Agi26k0gap Oligos for DpnII Restriction Sites[edit]

  • Lambda exonuclease digestion has been having low efficiency Try 1 and Try 2
    • Dr. Zhang suggested skipping lambda if very few oligos would be digested
  • Of 12,964 oligos, 8,744 (67%) have DpnII restriction sites besides the intended cleavage site
    • Conclusion: cannot skip lambda digestion step