Alice:LabNotes/2007-11-14

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Revision as of 22:48, 30 November 2007 by >Zsakura2
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I ran a gel of the PCR product from repeated experiment on sample #1,2,3,12,14,16. The gel image shows that all the reactions worked fine, so they can be prepared for sequencing. Eventually, the post PCR products are prepared with EXOSap.
File:ZhangLab 2 2007-11-14 17hr 10min.jpg

Also, I did PCR on sample #14 with newly designed primers rs1264899 along with template GC1EP, GC1F, GM20431, PGP1.
Protocol:

' one reaction total
Template 1 17
10x Buffer 5 85
10mM dNTP 1 17
10uM forward primer + reverse primer 1 17
50x SYBG I 0.4 6.8
Jump Start Tag DNA Polymerase 1 17
H2O 40.6 690.2

95C 3min -> 35 cycles of (95C 15S -> 58C 30S -> 72C 1min) -> 72C 3min

I forgot the reaction was only for 4 samples, therefore the left over master mix is stored in -20 fridge and saved for later use.