Hosuk:LabNotes/2014-7-8
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Deconvolution[edit]
- Try deconvolution with the sample(07/04; S1 : T48 RT primer, S2 : rHexamer), especially 63x Zoom=4 images
Procedure[edit]
- Plug-in of ImageJ --> PSF by 'PSF Generator’ : ‘PSF Gibson and Lanni_Zoom4_Z00.tif’
- Set parameter for 63x, zoom=4,
- 2048x2048, z=21, and use Z00
- Plug-in of ImageJ --> Deconvolution by ‘DeconvolutionJ’
Result[edit]
- Both images after deconvolution increase the number of rolony counted.
- Counts
- S1(T48 RT Primer) : 769 --> 907 (after deconvolution), 17.9% increase
- S2(random hexamer) : 1462 --> 1933 (after deconvolution), 32.3% increase