Hosuk:LabNotes/2014-7-8

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Revision as of 16:02, 8 July 2014 by >Hosuki78 (Created page with "*LabNote ===Deconvolution=== *Try deconvolution with the sample(07/04; S1 : T48 RT primer, S2 : rHexamer), especially 63x Zoom=4 images ====Procedure===...")
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Deconvolution[edit]

  • Try deconvolution with the sample(07/04; S1 : T48 RT primer, S2 : rHexamer), especially 63x Zoom=4 images


Procedure[edit]

  • Plug-in of ImageJ --> PSF by 'PSF Generator’ : ‘PSF Gibson and Lanni_Zoom4_Z00.tif’
    • Set parameter for 63x, zoom=4,
    • 2048x2048, z=21, and use Z00


  • Plug-in of ImageJ --> Deconvolution by ‘DeconvolutionJ’


Result[edit]

  • Both images after deconvolution increase the number of rolony counted.


  • Counts
    • S1(T48 RT Primer) : 769 --> 907 (after deconvolution), 17.9% increase
    • S2(random hexamer) : 1462 --> 1933 (after deconvolution), 32.3% increase


File:Deconv S1 Pos3.jpg


File:Deconv S2 Pos2.jpg