Hosuk:LabNotes/2014-7-9

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Revision as of 00:08, 10 July 2014 by >Hosuki78 (Created page with "*LabNote ===NCIH1975, PGP1F with T48 RT Primer=== *Fix NCIH1975 and PGP1F with 4% Formalin at 37C *S1 : T48 RT primer, S2 : rHexamer ====Result==== *Fix...")
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NCIH1975, PGP1F with T48 RT Primer

  • Fix NCIH1975 and PGP1F with 4% Formalin at 37C
  • S1 : T48 RT primer, S2 : rHexamer


Result

  • Fixing with 4% Formalin at 37C seems better in terms of even distribution of rolonies, which meaning less localized in nuclear
  • Rolony with T48 : 30~50% less than rolony with rHex


Tissue 11th

  • Tissue 11th: 10um thick, random hexamer, -a-dUTP


Result

Result : Tissue 11th: not as many as expected, similar to 9th result