Blue:RNA-Seq Analyses:C1 hNuc 1000 Dataset

From ZhangLabWiki
Jump to navigation Jump to search

Overview

  • C1 Run Summary:

File:20chips Samplelist.png


Average cDNA Yields Across Runs

File:20 C1 Runs cDNA Yields 2.png


  • Circled experiments represent the same batch of nuclei. There is little effect of freezing the nuclei in 10%DMSO or running the nuclei on a small chip rather than the medium chip. Global levels of cDNA were reduced with adding a nuclei stain/wash step to the loading protocol using the DNA Seq Cell Wash buffer. Also, addition of PolydIdC did globally increase cDNA levels even for the 0_Nuc conditions.