Blue:RNA-Seq Analyses:C1 hNuc 1000 Dataset
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Overview
- C1 Run Summary:
- Primary Mapping/Analysis done by Rui:LabNotes/SingleCell/2014-7-3 Rui using our pipeline
- More in depth analysis by Rui:LabNotes/SingleCell/2014-7-4 Rui
Average cDNA Yields Across Runs
File:20 C1 Runs cDNA Yields 2.png
- Circled experiments represent the same batch of nuclei. There is little effect of freezing the nuclei in 10%DMSO or running the nuclei on a small chip rather than the medium chip. Global levels of cDNA were reduced with adding a nuclei stain/wash step to the loading protocol using the DNA Seq Cell Wash buffer. Also, addition of PolydIdC did globally increase cDNA levels even for the 0_Nuc conditions.