Hosuk:LabNotes/2014-7-15

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Revision as of 05:36, 16 July 2014 by >Hosuki78
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20x obj. 4096 x 4096 image analysis

  • 1 pixel size = 141.91nm x 141.91nm
  • area_lower, area_upper : 6 ~ 50 (2.45 x 2.45 px ~ 7 x 7 px --> 347.68nm x 347.68nm ~ 994nm x 994nm)
  • PISA Param for 20x, 4096 x 4096
    • Gaussian Std : 2
    • Gaussian Upper : -5e-4
    • area upper: 50
    • area lower: 6
    • axratio lower: 0.5
    • circ upper: 1.6
    • circ lower: 0.8
    • perim conn: 8
    • bkgmult lower: 4