Matt:LabNotes/2014-7-17

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Revision as of 21:05, 17 July 2014 by >Mzcai (Created page with "==Agi26k0gap Probe Production== ===Production PCR=== *Make Master Mix for 46 well (6 strips) *0 gap first round amplicon -> V6 primers {| {{table}} | align="center" style="b...")
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Agi26k0gap Probe Production

Production PCR

  • Make Master Mix for 46 well (6 strips)
  • 0 gap first round amplicon -> V6 primers
Components 1x rxn (ul) 50x rxn (ul)
First round amplicon (10nM) 0.2 10
2x Kapa SYBR MM 50 2500
100uM pAP1V6U 0.4 20
100uM AP2V6 0.4 20
H2O 49 2450
Total 100 5000

98C 30sec -> (98C 10sec -> 55C 20sec -> 72C 30sec) x 15 -> 72C 2min -> 15C hold