Kun:LabNotes/Haplotyping/2009-1-29

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Revision as of 17:36, 29 January 2009 by KunZhang (talk | contribs) (New page: ==Perform 2nd round MDA on the amplicons from 01/28/2009== *The purpose of this experiment is to generate more DNA for downstream analyses. Only the 16 amplicons from the haploid templates...)
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Perform 2nd round MDA on the amplicons from 01/28/2009

  • The purpose of this experiment is to generate more DNA for downstream analyses. Only the 16 amplicons from the haploid templates will be re-amplified.
    Set up master mix:
                                         x 16
  10x RepliPhi phi-29 buffer     20ul     320
  1uM N6 primer                  10ul     160
  25mM dNTP                      8ul      128
  2X SYBR Green I                10ul     160
  H2O                           142ul    2272
  RepliPhi Phi-29 (100U/ul)      10ul      30
  Add 200ul MDA mix to each of the tube on cool block, 30C 2h, 85C 3min.