Kun:LabNotes/Haplotyping/2009-1-29
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Perform 2nd round MDA on the amplicons from 01/28/2009
- The purpose of this experiment is to generate more DNA for downstream analyses. Only the 16 amplicons from the haploid templates will be re-amplified.
Set up master mix: x 16 10x RepliPhi phi-29 buffer 20ul 320 1uM N6 primer 10ul 160 25mM dNTP 8ul 128 2X SYBR Green I 10ul 160 H2O 142ul 2272 RepliPhi Phi-29 (100U/ul) 10ul 30 Add 200ul MDA mix to each of the tube on cool block, 30C 2h, 85C 3min.