Daniel:Notebook/HiResChrPaint/2014-7-19
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Dystrophin Probes (Started 7/18/2014)
Dye Labeling
- Warm DMSO and bicarbonate labeling buffer and nuclease free water to room temperature
- Add 2.5 uL DNA and 1.5 uL of sodium bicarbonate buffer
- Denature samples for 5 minutes at 95C, then snap cool using ice box
- Dissolve 1 vial of reactive dye in 2 uL solvent; vortex ≥10 seconds
- dye cannot be saved for later use. Use immediately!
- Add 1 uL dye to sample
- Incubate in the dark for 1 hour
- Add 5 uL 3M NaOAc and 40 uL nfH20 to sample
- Recommend column purification after incubation