Daniel:Notebook/HiResChrPaint/2014-7-19

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Revision as of 19:56, 19 July 2014 by >Djacobse (Created page with "=Dystrophin Probes (Started 7/18/2014)= Back to Calendar ==Dye Labeling== #Warm DMSO and bicar...")
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Dystrophin Probes (Started 7/18/2014)

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Dye Labeling

  1. Warm DMSO and bicarbonate labeling buffer and nuclease free water to room temperature
  2. Add 2.5 uL DNA and 1.5 uL of sodium bicarbonate buffer
  3. Denature samples for 5 minutes at 95C, then snap cool using ice box
  4. Dissolve 1 vial of reactive dye in 2 uL solvent; vortex ≥10 seconds
    1. dye cannot be saved for later use. Use immediately!
  5. Add 1 uL dye to sample
  6. Incubate in the dark for 1 hour
  7. Add 5 uL 3M NaOAc and 40 uL nfH20 to sample
  8. Recommend column purification after incubation