Hosuk:LabNotes/2014-7-20
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Deconvolution and PSF images - Update
PSF Functions
- Generated by PSF Generator plug-in
- Settings
File:PSFGeneratorSettings 2014-07-20.png.png
- PSF_20x_4096x4096_best1st_40x40.tif
- PSF_63x_2048x2048_best1st_40x40.tif
- PSF_63xZoom4_2048x2048_best1st_128x128.tif.tif
Procedure for PISA
- Pre-processing before an actual identification algorithm
- img_raw = imread(ImgFileName);
- img_double1 = mat2gray(img_raw);
- [img_decv, PSF] = deconvblind(img_double1, PSFimg, 10);
- img_flt = medfilt2(img_decv, [3 3]);
- [xsize, ysize]=size(img_raw);
- hi = prctile( double( reshape(img_flt, 1, xsize*ysize) ), 99.9);
- img_double = double(img_flt)/hi;
- Need to test the result between doing medfilt2 or not for 20x 4096x4096
- medfilt2 (of 20x 4096x4096 image) seems to make more blurred image…