Matt:LabNotes/2014-8-4

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Revision as of 22:05, 4 August 2014 by >Mzcai (Created page with "==Agi26k0gap Probe Production Continued== *Continued from: Matt:LabNotes/2014-7-22 **Thaw and combine the 7 tubes of 200ul that were stored at -20C ===TBU Gel Check=== *2...")
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Agi26k0gap Probe Production Continued

TBU Gel Check

  • 2ul Sample + 2ul 2X Urea Loading Dye
  • 0.5ul Low Mass Ladder + 1.5ul H2O + 2ul 2X Urea Loading Dye
  • Digested sample
  • Undigested sample: 10nM 1st round amplicon

[[]]

  • Brightest band below 200bp as expected

Column Purification

  • Used Zymo ssDNA/RNA columns
  • Total Eluted: ul
  • Nanodrop quant: ng/ul
  • Yield: ng/ul x ul = ug -->