Arichard:Notebook/bulkNxt2014Jul30

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Revision as of 18:58, 21 August 2014 by >Andrew (→‎Sequencing statistics)
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Bulk Nextera libraries from neuronal nuclei

Neuronal nuclei were sorted May 20, 2014 by Gwen Kaeser from AD patient 25-00 and control patient 703. Both were females in their 80s. Both cortex and cerebellum were sorted for each patient. Nuclei were sorted from the Hi DCV (Hi PI staining for DNA) fraction of each peak. Sort conditions were +/- for NeuN.

  • 25-00 Ctx+
  • 25-00 Ctx-
  • 25-00 Cbl+
  • 7-03 Ctx+
  • 7-03 Ctx-
  • 7-03 Cbl+

~1200 nuclei were used for each sample. Hypotonic lysis buffer was used. TO BE CONTINUED.

Gels after Nextera PCR

13 total cycles.

File:BulkNxt2014Jul30GelsSlide1.JPG

File:BulkNxt2014Jul30GelsSlide2.JPG

File:BulkNxt2014Jul30GelsSlide3.JPG

File:BulkNxt2014Jul30GelsSlide4.JPG

Sequencing statistics

Sequencing statistics for bulk Nextera libraries from May 20 sort