Hosuk:LabNotes/2014-8-22

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Revision as of 22:26, 22 August 2014 by >Hosuki78 (Created page with "*LabNote ===Decoding with Agi26k0gap Padlock Probe : Masking images with MIP of all steps=== ====Masking==== *Done by Matt *MIP with images of all steps ...")
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Decoding with Agi26k0gap Padlock Probe : Masking images with MIP of all steps

Masking

  • Done by Matt
  • MIP with images of all steps
  • Run PISA of the MIP

seg_data = regionprops(MaskedFile,MaskedImgFile, 'MeanIntensity', 'Area', 'Centroid');


  • Image resolution : 20x obj, 4096 x 4086, z step = 1.04um
    • Since how many features would be detected or how much dense rolonies in this sample, so image in wide field of view
    • eventually 63x obj.