AlanFung:LabNotes/2014/2014-9-5

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Revision as of 23:06, 4 September 2014 by >Alan6017518 (→‎Samples)
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KAPA Library Preparation Kit illumina Platforms

Parts

  • Kapa Library Preparation Kit illumina platforms KR0935 V1.13

Samples

abc def ghi jkl
Sample Amount Qubit ng/ul 10 100 Water Condition
6T1 100ng 43.4 2.30 47.7 Sheared
6T2 100ng 31.6 3.16 46.8 Sheared
6T3 100ng 31.5 3.17 46.8 Sheared
6T4 100ng 41.7 2.40 47.6 Sheared
6T5 100ng 30.5 3.28 46.7 Sheared
7T1 100ng 30.7 3.26 46.7 Sheared
7T2 100ng 44.6 2.24 47.8 Sheared
7T3 100ng 35.9 2.79 47.2 Sheared
7T4 100ng 33.8 2.96 47.0 Sheared
7T5 100ng 20.4 4.90 45.1 Sheared
PCT1 100ng 82.7 1.21 48.8 Sheared
PCT2 100ng 35.8 2.79 47.2 Sheared
PCT4 100ng 42.8 2.34 47.7 Sheared
PCT6 100ng 15.2 6.58 43.4 Sheared
PCT7 100ng 16.3 6.13 43.9 Sheared
NORM1-02 P1 10ng 1.66 6.02 43.98 non-Sheared
NORM1-03 P1 10ng 0.77 12.99 37.01 non-Sheared
COLO2-03 10ng 1.11 9.01 40.99 non-Sheared
cfDNA 10ng 7.09 1.41 48.59 non-Sheared

Workflow

End Repair Reaction Setup

Component 1 Library 15 Libraries + 5%
End Repair Master Mix:    
Water 8 126
10X Kapa End Repair Buffer 7 110.25
Kapa End Repair Enzyme Mix 5 78.75
Total master mix volume 20 315
Fragmented, double-stranded DNA 50ul
End Repair Master Mix 20ul
Total rection Mix 70ul
  • Mix, and incubate at 20C for 30mins
  • Proceed immediately to the next step

End Repair Cleanup