Hosuk:LabNotes/2014-9-5
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PGP1F 2nd rolonyar20140414 – Decode again with 63x
- 63x, zoom=1, 2x2 tile (each tile index : s0~s3, --> converted to s1~s4 for analysis)
- Z=0.49um, ~22 images
Result
- Image signal were too saturated… need to use less PMT gain… (this time it was 660, 4% laser)
Merged, Tile, BF
- File:GeneMapTable IntPlot s1.png
- File:GeneMapTable IntPlot s2.png
- File:GeneMapTable IntPlot s3.png
- File:GeneMapTable IntPlot s4.png